- Product Details
Keywords
Quick Details
- ProName: URB-597
- CasNo: 546141-08-6
- Molecular Formula: C20H22N2O3
- Appearance: white crystal powder
- Application: PLANTOR RESEARCH
- DeliveryTime: 3 DAYS
- PackAge: discreet
- Port: EMS
- ProductionCapacity: /
- Purity: 99.5%
- Storage: dry
- Transportation: AIR
- LimitNum: 1 Kilogram
Superiority
Membranefractionsarepreparedfrombrainhomogenates,andFAAHactivityisassayedusing[3H]anandamide(anandamide[ethanolamine-3H],60Ci/mmol)asasubstrate.Ratbra…
Details
Membrane fractions are prepared from brain homogenates, and FAAH activity is assayed using [3H]anandamide (anandamide[ethanolamine-3H], 60 Ci/mmol) as a substrate. Rat brain membranes (50 μg protein) are incubated for 30 min at 37 °C in buffer containing [3H]anandamide and varying concentrations of URB597. At the end of the incubation period, we stopp the reactions with a mixture of chloroform/methanol and measured [3H]ethanolamine in the aqueous phase by liquid scintillation counting.